Fluorescein-4-isothiocyanate (FITC-II)

Fluorescein-4-isothiocyanate (FITC-II) - Names and Identifiers

Name	fluorescein isothiocyanate isomer ii
Synonyms	6-FITC FITC ISOMER II FITC 'ISOMER II' Fluorescein isothioc 6-ISOTHIOCYANATO-FLUORESCEIN FLUORESCEIN-6-ISOTHIOCYANATE 6-fluorescein isothiocyanate fluorescein isothiocyanate isomer ii FLUORESCEIN 6-ISOTHIOCYANATE, ISOMER 2 FLUORESCEIN ISOTHIOCYANATE (ISOMER II) Fluorescein-4-isothiocyanate (FITC-II) Fluorescein-6-isothiocyanate (FITC Isomer II) 2-(6-HYDROXY-3-OXO-3H-XANTHEN-9-YL)-4-ISOTHIOCYANATO-BENZOIC ACID 3',6'-dihydroxy-6-isothiocyanato-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one 3',6'-Dihydroxy-6-isothiocyanatospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
CAS	3012-71-3 18861-78-4 3326-31-6
EINECS	248-207-7
InChI	InChI=1/C21H11NO5S/c23-12-2-5-15-18(8-12)26-19-9-13(24)3-6-16(19)21(15)17-7-11(22-10-28)1-4-14(17)20(25)27-21/h1-9,23-24H

Fluorescein-4-isothiocyanate (FITC-II) - Physico-chemical Properties

Molecular Formula	C21H11NO5S
Molar Mass	389.38
Density	1.54g/cm³
Boling Point	719.1°C at 760 mmHg
Flash Point	388.7°C
Vapor Presure	2.28E-21mmHg at 25°C
Appearance	lyophilized powder
Storage Condition	2-8°C
Refractive Index	1.753

Fluorescein-4-isothiocyanate (FITC-II) - Risk and Safety

Hazard Symbols	Xi - Irritant
Risk Codes	R36 - Irritating to the eyes R42/43 - May cause sensitization by inhalation and skin contact.
Safety Description	S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S45 - In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.) S36/37 - Wear suitable protective clothing and gloves. S22 - Do not breathe dust.
WGK Germany	3
HS Code	29329990

Fluorescein-4-isothiocyanate (FITC-II) - Reference

Reference

1. [IF=6.953] Dong Yu-Hao et al."Study on the pharmacokinetics of mulberry fruit polysaccharides through fluorescence labeling."Int J Biol Macromol. 2021 Sep;186:462
2. [IF=4.952] Feng Xue et al."Prevention of frozen-dough from deterioration with incorporation of glutenin-polyphenols conjugates prepared by ultrasound."Lwt Food Sci Technol. 2021 Nov;151:112141

Fluorescein-4-isothiocyanate (FITC-II) - Introduction

fluorescein isocyyanate isomer ii(Fluorescein isocyyanate isomer II) is a fluorescent dye. The following is a description of its nature, use, preparation and safety information:

1. Nature:
-chemical formula: C21H11O5N2S
-Molecular weight: 389.39g/mol
-Appearance: Yellow crystalline powder
-Solubility: Soluble in organic solvents, such as dimethyl sulfoxide (DMSO), Dimethyl Formamide (DMF), etc.
-Fluorescence characteristics: Under ultraviolet light (excitation wavelength is about 490 nm), the fluorescence emission peak is about 520 nm

2. Use:
-Cell and molecular biology research: can be used for chromosome markers, protein, antibody or nucleic acid coupling, and intracellular and intercellular tracing
-Immunofluorescence staining: suitable for immunohistochemical staining and flow cytometry
-Fluorescent probes: widely used in biological imaging and fluorescence microscopy research

3. Preparation method:
The general preparation method is synthesized by reacting fluorescein with isothiocyanate. Specific preparation methods may vary, but the main steps include obtaining fluorescein acid, which is then reacted with isothiocyanate to form fluorescein isothiocyanate isomer ii.

4. Safety Information:
- fluorescein isothiocyanate isomer ii may release toxic fumes under flammable conditions.
-Use with care to avoid contact with skin, eyes and taking.
-Wear appropriate protective equipment such as lab gloves, goggles and lab coats during operation.
-storage should be sealed in a cool and dry place, away from fire and high temperature.
-Detailed safety information can be obtained in the product manual or in the safety data sheet provided by the supplier.

Last Update：2024-04-10 22:29:15